These data suggest that the abattoir type may not be as important in the prevalence and spread of STEC as the hygiene practices of the workers. coli in samples collected from butchering knife was significantly lower when compared with that of the workers’ hand (P = 0.047) and slaughtering floor (P = 0.047) but not with the slaughter table (P = 0.98) and effluent water from the abattoir house (P = 0.39). Results showed that the average cfu/cm 2 was significantly lower in the samples collected at Sekona-2 slaughter slab compared with those collected at Al-maleek batch abattoir and Sekona-1 slaughter slab in ascending order at P = 0.03. Sequence analysis of PCR products was performed on a representative isolate showing the highest combination of virulence genes using the 16S gene for identification purposes only. These were confirmed serologically as non-O157 STEC using latex agglutination serotyping kit. Multiplex PCR analysis was used to screen for the presence of specific virulence factors. Samples were plated directly on selective and differential media and E. coli (STEC) known to be etiological agents for diarrhea were screened for their incidence/occurrence in selected abattoirs sources in Osogbo metropolis of Osun State, Nigeria using a randomized block design.

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